Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients

Dan Wu; Lixing Liu; Min Zhang; Xianhui Min

doi:doi:10.11648/j.cmr.20221102.13

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Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients

Dan Wu, Lixing Liu, Min Zhang, Xianhui Min

Published in Clinical Medicine Research (Volume 11, Issue 2)

Received: 4 March 2022 Accepted: 21 March 2022 Published: 14 April 2022

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Abstract

Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.

Published in	Clinical Medicine Research (Volume 11, Issue 2)
DOI	10.11648/j.cmr.20221102.13
Page(s)	28-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Genistein, Preeclampsia, Human Umbilical Vein Endothelial Cells, Oxidative Stress, Anti-apoptosis

References

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APA Style

Dan Wu, Lixing Liu, Min Zhang, Xianhui Min. (2022). Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clinical Medicine Research, 11(2), 28-35. https://doi.org/10.11648/j.cmr.20221102.13

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ACS Style

Dan Wu; Lixing Liu; Min Zhang; Xianhui Min. Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clin. Med. Res. 2022, 11(2), 28-35. doi: 10.11648/j.cmr.20221102.13

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AMA Style

Dan Wu, Lixing Liu, Min Zhang, Xianhui Min. Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clin Med Res. 2022;11(2):28-35. doi: 10.11648/j.cmr.20221102.13

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@article{10.11648/j.cmr.20221102.13,
  author = {Dan Wu and Lixing Liu and Min Zhang and Xianhui Min},
  title = {Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients},
  journal = {Clinical Medicine Research},
  volume = {11},
  number = {2},
  pages = {28-35},
  doi = {10.11648/j.cmr.20221102.13},
  url = {https://doi.org/10.11648/j.cmr.20221102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20221102.13},
  abstract = {Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.},
 year = {2022}
}

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TY  - JOUR
T1  - Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients
AU  - Dan Wu
AU  - Lixing Liu
AU  - Min Zhang
AU  - Xianhui Min
Y1  - 2022/04/14
PY  - 2022
N1  - https://doi.org/10.11648/j.cmr.20221102.13
DO  - 10.11648/j.cmr.20221102.13
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 28
EP  - 35
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20221102.13
AB  - Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.
VL  - 11
IS  - 2
ER  -

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Author Information

Dan Wu

Department of Obstetrics and Gynecology, Putian University, Putian, China
Lixing Liu

Department of Obstetrics and Gynecology, Putian University, Putian, China
Min Zhang

Department of Obstetrics and Gynecology, Putian University, Putian, China
Xianhui Min

Department of Obstetrics and Gynecology, Clinical Division, 900 Hospital of PLA Department of General Logistics, Fuzhou, China

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Plain Text BibTeX RIS

APA Style

Dan Wu, Lixing Liu, Min Zhang, Xianhui Min. (2022). Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clinical Medicine Research, 11(2), 28-35. https://doi.org/10.11648/j.cmr.20221102.13

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ACS Style

Dan Wu; Lixing Liu; Min Zhang; Xianhui Min. Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clin. Med. Res. 2022, 11(2), 28-35. doi: 10.11648/j.cmr.20221102.13

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AMA Style

Dan Wu, Lixing Liu, Min Zhang, Xianhui Min. Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients. Clin Med Res. 2022;11(2):28-35. doi: 10.11648/j.cmr.20221102.13

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@article{10.11648/j.cmr.20221102.13,
  author = {Dan Wu and Lixing Liu and Min Zhang and Xianhui Min},
  title = {Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients},
  journal = {Clinical Medicine Research},
  volume = {11},
  number = {2},
  pages = {28-35},
  doi = {10.11648/j.cmr.20221102.13},
  url = {https://doi.org/10.11648/j.cmr.20221102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cmr.20221102.13},
  abstract = {Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.},
 year = {2022}
}

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TY  - JOUR
T1  - Genistein Improves Barrier Function and Proliferation of Human Umbilical Vein Endothelial Cells from Preeclampsia Patients
AU  - Dan Wu
AU  - Lixing Liu
AU  - Min Zhang
AU  - Xianhui Min
Y1  - 2022/04/14
PY  - 2022
N1  - https://doi.org/10.11648/j.cmr.20221102.13
DO  - 10.11648/j.cmr.20221102.13
T2  - Clinical Medicine Research
JF  - Clinical Medicine Research
JO  - Clinical Medicine Research
SP  - 28
EP  - 35
PB  - Science Publishing Group
SN  - 2326-9057
UR  - https://doi.org/10.11648/j.cmr.20221102.13
AB  - Preeclampsia (PE), which is characterized by hypertension in women who have normal blood pressure before pregnancy and is accompanied by proteinuria, edema, and major organ damage, is the major cause of the increased mortality in pregnant women and perinatal fetuses. So far, genistein is recognized as the only safe and effective natural phytoestrogen without estrogen-like adverse reactions. Recent studies show that genistein may play a significant role in controlling oxidative/nitrative stress during preeclampsia, indicating a possible beneficial role of genistein in the prevention of preeclampsia. However, the ability of genistein to prevent and treat pregnancy-induced hypertension, especially preeclampsia, is unknown. To study the effect of genistein on endothelial cell damage in preeclampsia, we isolated human umbilical vein cells (HUVEC) from 20 normal pregnant women and 40 preeclampsia patients (half treated with genistein and the other half untreated). We found that HUVEC barrier function, proliferation, nitric oxide synthesis, glutathione peroxidase activity, and Bcl-2 expression were significantly decreased in the PE group. Furthermore, 8-hydroxy-2’-deoxyguanosine levels and Bax expression were significantly increased comparing to control group. Genistein treatment reversed these changes in the PE group, suggesting that genistein reduces endothelial cell damage in preeclampsia HUVEC and providing a supporting the use of genistein for PE prevention and treatment.
VL  - 11
IS  - 2
ER  -

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