Volume 8, Issue 1, January 2019, Page: 21-26
MiR203 Lost Suppressor Genes Function in the Process of Barrett’s Esophagus Carcinogenesis Because of High Methylation in Promoter
Liu Tianyu, Department of Digestion and Gastroenterology, Suining Central Hospital Affiliated to Chongqing Medical University, Suining, China
Long Xiaoqi, Department of Digestion and Gastroenterology, Suining Central Hospital Affiliated to Chongqing Medical University, Suining, China
Received: Nov. 13, 2018;       Accepted: Jan. 11, 2019;       Published: Apr. 1, 2019
DOI: 10.11648/j.cmr.20190801.14      View  109      Downloads  15
Abstract
This study was designed to explore the role of MiR203 promoter methylation in the process of Barrett’s esophagus carcinogenesis. RT-PCR was used to detect the expression levels of miRNA-203 in Barrett’s esophagus, esophageal cancer and normal esophageal mucosa cell lines, before and after the treatment of demethylation. MiR203 promoter methylation levels in these cell lines were measured by Methylation Specific PCR (MSP). Immunohistochemistry was used to test the expression and distribution of K-Ras, a target of miR203, in esophageal cancer, BE and normal esophagus tissues. The following results were found based on the above methods. MiR203 expression levels were reduced obviously in Barrett esophagus and esophageal cancer cells than normal esophageal cells, the difference was statistically significant (P=0.003). After demethylation treatment, miR203 expression levels were significantly increased in Barrett's esophagus and esophageal cancer cells, the differences were statistically significant (P=0.03). MSP results showed that miR203 promoter changed to be low-methylation or non-methylation after demethylation treatment. In conclusion, MiR203 in Barrett's esophagus and esophageal cancer cells reduced expression is related to its Promoter methylation, miR203 promoter methylation throughout the carcinogenesis of Barrett's esophagus, it may become a key molecular biomarker in process of Barrett esophagus cancerous, and may become the prevention and treatment targets of Barrett esophagus carcinogenesis.
Keywords
Barrett's Esophagus, miR203, Methylation, Esophagus Carcinogenesis
To cite this article
Liu Tianyu, Long Xiaoqi, MiR203 Lost Suppressor Genes Function in the Process of Barrett’s Esophagus Carcinogenesis Because of High Methylation in Promoter, Clinical Medicine Research. Vol. 8, No. 1, 2019, pp. 21-26. doi: 10.11648/j.cmr.20190801.14
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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